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KMID : 0371320020620020103
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Journal of the Korean Surgical Society
2002 Volume.62 No. 2 p.103 ~ p.111
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Study of Capsaicin-induced Apoptosis in Human Colon Cancer Cell Lines
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Kim Kyu-Yeol
Yang kyung-Min
Seong Moo-Kyung
Park Ung-Chae
Choi Dae-Hwa
Nam Chang-Woo
Nah Yang-Won
Ko Byung-Kyun
Park Kun-Choon
Lim Young-Chul
Kim Byung-Sam
Cho Hong-Rae
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Abstract
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Purpose: Numerous investigations have been conducted in order to
determine the potential carcinogenic or chemopreventive activity of
capsaicin. The aim of this study is to characterize the effects of capsaicin
on colon cancer cells, and provide valuable information concerning the
application of capsaicin in chemoprevention as well as for therapeutic
purposes. Methods: CoLo320DM and LoVo cells (human colon cancer cell
line) were treated with capsaicin. In order to access cell viability and
altered morphology, an MTT assay was performed and the cells were
microscopically examined. Decreasing DNA staining was accessed by FACS. The
cells were stained with FITC labeled annexin V and analyzed by FACS to
detect cellular membrane alteration during apoptosis. The cells were stained
with DiOC6(3) and Hydroethidine and analyzed by FACS in order to access ROS
and ΔΨm. Results: Capsaicin decreased cell viability in
a dose-dependent manner. Capsaicin produced a cell morphology corresponding
to the apoptotic features including cell shrinkage and chromatic
condensation. Capsaicin treated cells induced a loss of nuclear DNA leading
to hypoploidy in a dose-dependent manner. Cells were excluded by double
staining with PI and FITC labeled annexin v and detected by FACS. We show
that treatment of CoLo320DM, L0Vo cells with increasing concentrations of
capsaicin parallel an increase in the percentage of red fluorescent cells
(HE→Eth) that reflect ROS hypergeneration and a decrease in the percentage
of green fluorescent cells that reflect ΔΨm disruption.
Conclusion: These results clearly demonstrate that capsaicin-induced
colon cancer cell death is apoptotic.
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KEYWORD
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